ig® Recombinase Polymerase Amplification (RPA) Kit
IG® Recombinase Polymerase
Amplification (RPA) Kit provides a mixture of the necessary enzymes to simplify
and quicken the setup for your RPA reaction.
Description
The ig® RPA
Kit from Intact Genomics gives the user a faster, more streamlined Recombinase
Polymerase Amplification experience by providing the necessary reagents and
enzymes to amplify user-provided DNA template. Isothermal RPA is an enhancement
over PCR amplification because it does not require thermocycling and provides
better sensitivity.
Recombinase Polymerase
Amplification (RPA) is a molecular biology technique that amplifies DNA at a
constant temperature (37-42°C) using a recombinase (e.g.
UvsX),
primers, a single-stranded DNA binding protein (SSB), and a strand displacing
DNA polymerase. T4 UvsX is
used in combination with its accessory protein, UvsY.
The recombinase interacts with the primers to form nucleoprotein filament. This
complex can bind with homologous double-stranded DNA through a strand exchange.
After the exchange, a single-stranded binding protein, T4 gp32, stabilizes the
displaced strand. Finally, Bsu/Sau
DNA polymerase extends the DNA from the primers and creates a new complete copy
of the template. Amplification can continue as in Polymerase Chain Reaction
(PCR).
Components and Storage
•Enzyme
Mixture
•2x
RPA Reaction Master Mix
•280
mM Magnesium Acetate (MgOAc)
Storage Temperature
-20°C
Schematic of Recombinase
Polymerase Amplification
Related Products
1.T4
UvsX
Recombinase (Cat.# 3562) 2.T4
gp32 Protein (Cat.# 3515) 3.T4
UvsY
Protein (Cat.# 3572) 4.Bsu
DNA Polymerase (Cat.# 3585) 5.Sau
DNA Polymerase (Cat.# 3595) 6.Exonuclease
III (Cat.# 3415)
7.Exonuclease
IV (Nfo)
(Cat.# 3425)
Technical Support
Intact Genomics is committed to
supporting the worldwide scientific research community by supplying the highest
quality reagents. Each new lot of our products is tested to ensure it meets the
quality standards and specifications designated for the product.
Please follow the instructions
carefully and contact us if additional assistance is needed. We appreciate your
business and your feedback regarding the performance of our products in your
applications.
