Methionine Auxotrophic Agrobacteria 

Competent Cells

Our new Methionine Auxotrophic Agrobacterium have been engineered to utilize methionine, an essential amino acid, for growth and survival. Methionine Auxotrophic Agrobacterium’s growth is halted unless free methionine is added to minimal medium.  

Successful transformation often depends on the ability to control Agrobacterium using antibiotics to prevent it from overgrowing the explants, however antibiotics can have undesired effects on the plant tissue. The use of Auxotrophic Agrobacterium minimize this issue and their modifications simplify procedures and increase overall plant transformation efficiency.  Ultimately, the use of autotrophs reduces bacterial overgrowth during co-cultivation during plant transformation. 

Benefits Include:

●  Enables the development of more efficient transformation systems

●  Reduced bacterial overgrowth during co-cultivation
●  Decreased need for antibiotics

Methionine Auxotrophic Agrobacterium Competent Cells 
Need more information?  Please find the publication from the University of Georgia as well as our product link below.

Prías-Blanco, M., Chappell, T.M., Freed, E.F. et al. An Agrobacterium strain auxotrophic for methionine is useful for switchgrass transformation. Transgenic Res 31, 661–676 (2022). https://doi.org/10.1007/s11248-022-00328-4