Isothermal Nucleic Acid
Amplification made easier with one simple kit.
Intact
Genomics is a global leader in
research and development of RPA technology and enzymes. We are introducing the ig® Recombinase
Polymerase Amplification (RPA) Kit which gives the user a faster, more
streamlined RPA experience by providing the necessary reagents and enzymes to
amplify user-provided DNA template. Isothermal RPA is an enhancement over PCR
amplification because it does not require thermocycling.
Description
The ig® RPA
Kit from Intact Genomics gives the user a faster, more streamlined Recombinase
Polymerase Amplification experience by providing the necessary reagents and
enzymes to amplify user-provided DNA template. Isothermal RPA is an enhancement
over PCR amplification because it does not require thermocycling and provides
better sensitivity.
Recombinase Polymerase
Amplification (RPA) is a molecular biology technique that amplifies DNA at a
constant temperature (37-42°C) using a recombinase (e.g.
UvsX),
primers, a single-stranded DNA binding protein (SSB), and a strand displacing
DNA polymerase. T4 UvsX is
used in combination with its accessory protein, UvsY.
The recombinase interacts with the primers to form nucleoprotein filament. This
complex can bind with homologous double-stranded DNA through a strand exchange.
After the exchange, a single-stranded binding protein, T4 gp32, stabilizes the
displaced strand. Finally, Bsu/Sau
DNA polymerase extends the DNA from the primers and creates a new complete copy
of the template. Amplification can continue as in Polymerase Chain Reaction
(PCR).
Components and Storage
•Enzyme
Mixture
•2x
RPA Reaction Master Mix
•280
mM Magnesium Acetate (MgOAc)
Storage Temperature
-20°C
