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[Intact Genomics] Recombinase Polymerase Amplification

관리자 2023.03.23 14:26 조회 2097 추천 18
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RPA, is it a good fit for your research?


Find out if RPA is right for your research and how to use it to potentially improve your end results via isothermal activity. Our newest resource takes a look into getting started with RPA, its benefits, and its general uses. Our Scientists have curated an informative new resource including multiple publications for those who are considering trying RPA.


What is RPA?

 

Recombinase Polymerase Amplification (RPA) is an enzyme- based alternative to Polymerase Chain Reaction (PCR) that amplifies isothermally. Like PCR, the primary objective of RPA is to make abundant copies of a targeted DNA sequence. Both PCR and RPA use a set of primers that target a DNA sequence of interest and a polymerase to make copies of that DNA sequence. RPA also achieves max amplification quicker than PCR.

 

We supply several publications and elaborate on the history and basic principles of this isothermal enzyme-based alternative to Polymerase Chain Reaction further in the article. Click below to read the full article and resources. 



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Figure 1: Representation of the structure of an RPA polymerase (PDB ID: 2HHS) with bound DNA. 

3' Nuclease domain is purple, polymerase domain is blue, DNA is depicted as an orange helix.


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Figure 2: Ribbon representation of UvsX (PDB ID: 3IO5), a DNA recombinase enzyme.




What Enzymes are needed?

 

We discuss the four enzymes needed (T4 UvsX DNA Polymerase, T4 UvsY Protein, T4 gp32 and Bsu DNA Polymerase) and their roles in the reaction.

 

Our team compiled over 30 publications that cover the history of RPA, uses of RPA, and options for optimization of RPA.

 

To view the full in-depth article and to learn more click the button below: 



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Intact Genomics RPA Related Products


 Product Link Package Size Features
 T4 UvsX DNA Recombinase 100 μg, 500 μg, & 1000 µg Important for the error-free repair of DNA double-strand breaks and for replication fork restart.
 T4 UvsY Protein 100 μg, 500 μg, & 1000 µg T4 UvsY is the phage T4 recombination mediator protein. It stimulate sthe filament nucleation event.
 T4 gp32 Protein 500 μg Single-stranded DNA binding protein required for T4 DNA replication, recombination, and repair.
 Klenow Fragment exo- (3’→5′ exo-) 1,000 units Lacks the N-terminal 324 amino acids.
 Bsu DNA Polymerase Large Fragment 1,000 units Polymerase useful for extending primers in RPA.
 Sau DNA Polymerase Large Fragment 1,000 units Polymerase useful for extending primers in RPA.




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