96-Well Plate
Target Name
HSD11b1 / 11-Beta-Hydroxysteroid
Dehydrogenase Type 1
Target Synonyms
11DH; 11-beta-HSD1; HDL; HSD11;
HSD11B; HSD11L; SDR26C1; Corticosteroid 11-beta-dehydrogenase isozyme 1; Short
Chain Dehydrogenase/Reductase Family 26C,Member 1
Species Reactivity
Human
Sample Type
Tissue homogenates, cell lysates
and other biological fluids
Detection Range
1.56-100ng/mL
Sensitivity
0.56ng/mL
Estimated Turnaround Time
7-11 business days
Precision
Intra-assay Precision (Precision
within an assay): 3 samples with low, middle and high level
11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) were tested 20 times on
one plate, respectively. Inter-assay Precision (Precision between assays): 3
samples with low, middle and high level 11-Beta-Hydroxysteroid Dehydrogenase
Type 1 (HSD11b1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%
Detection Method
Sandwich
Assay Time
3 hours
Application
ELISA
Assay Type
Sandwich
Shipping Condition
Ice Packs
Storage
4°C for 1 month/ -20°C for
long-term(within shelf life)
Precaution of Use
The Stop Solution is acidic. Do
not allow to contact skin or eyes.
Expiry Date
8 months
UniProt
ID
P28845
Specificity
This assay has high sensitivity
and excellent specificity for detection of 11-Beta-Hydroxysteroid Dehydrogenase
Type 1 (HSD11b1). No significant cross-reactivity or interference between
11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) and analogues was
observed.
Stability
The stability of kit is
determined by the loss rate of activity. The loss rate of this kit is less than
5% within the expiration date under appropriate storage condition. To minimize
extra influence on the performance, operation procedures and lab conditions,
especially room temperature, air humidity, incubator temperature should be
strictly controlled. It is also strongly suggested that the whole assay is
performed by the same operator from the beginning to the end.
Restrictions
For Research Use Only. Not for
use in diagnostic procedures. Not for human or animal drug or food use.
Quality Assurance
Biomatik
ELISA Kits are not typically pre-assembled as finished products due to limited
shelf life. Before shipping, each kit is assembled and tested to ensure that it
meets specifications before shipping. Minor changes may occur to the Detection
Range, Sensitivity, and Precision. All kits are tested to confirm that they
fall within their defined Inter- and Intra- assay coefficient of variation.
Please always refer to the hard copy manual included in the kit before
experiment.
Research Area
Enzyme & Kinase;Metabolic
pathway;Endocrinology;Cardiovascular
biology;Neuro
science;Hepatology;Hormone
metabolism;
Highlights of ELISA Kits from Biomatik
1. High sensitivity and low
detection range: Our lowest detection ranges are in the picogram range
(1-100pg/mL, 3-200pg/mL).
2. A wide range of sample types: Serum, plasma, tissue homogenates, cell
lysates, cell culture supernatants, and other biological fluids.
3. Enhanced specifications: Some of our kits feature a shortened experimental
time, pre-diluted detection reagents, and enhanced stability.
4. Customer support: Thorough technical support - we stand behind our products
and assist our customers promptly whenever the need arises.
