Introducing Our Full Line Up

We’re bringing you our new gene editing products this fall for Knockin/Knockout/Prime-editing experiments. At IG® we’re laser focused on innovation for your gene editing and cloning needs. Come check it out! 

IG® sgRNA Synthesis Kits for Cas9 or Cas12 Nuclease

IG® sgRNA Synthesis Kits can be used to quickly synthesize sgRNA by in vitro transcription enabling larger amounts of RNA than conventional kits.

Cas9 Nuclease

Intact Genomics was one of the earliest suppliers of Cas9 Nuclease. Cas9 Nuclease is the purified recombinant Streptococcus pyogenes Cas9 enzyme. Our Cas9 nuclease contains a nuclear localization signal (NLS) and displays excellent activity and purity. Cas9 cuts both strands of DNA creating a blunt end break. 

Cas12a

Our Cas12a Nuclease is the purified recombinant Francisella tularensis subsp. novicida Cas12a enzyme for gene editing. In contrast to blunt-end dsDNA breaks by Cas9 nuclease, Cas12a cleaves the DNA creating “sticky” end dsDNA breaks with a four base pair overhang. Cas12a has more specificity for the target sequence by having longer stretches of mismatch detection of gRNA-target DNA in gene editing.

IG® RNA Cleanup Kit 

A quick and easy protocol to purify and concentrate up to 50 µg of RNA from enzymatic reactions. The IG® RNA Cleanup Kit is recommended following the IG® sgRNA Synthesis Kit procedure to remove proteins, salts, and most unincorporated nucleotides for downstream use of the in vitro transcribed sgRNA.