Protein–Oligo Conjugation Kit
(S-9011-1)
Description
The
Protein-Oligo Conjugation Kit is designed to easily and efficiently prepare two
separate protein-oligo conjugates. This kit is flexible so that researchers
with little or no conjugation experience can make their own custom
protein-oligo conjugates to suit their research needs. It includes all
of the necessary components, including S-HyNic
and S-4FB, for the rapid and specific crosslinking of any protein with any
oligo from 20 to 100 nucleotides in length.
The molecular weight range for proteins to be conjugated with this kit is
25,000 to 900,000 Daltons. Protein amounts from 50 – 650 µg at a concentration
of 1 – 5 mg/mL in a volume of 50 – 130 µL are suitable for conjugation.
Specifications
Kit Components
•S-HyNic
(2 X 1 mg)
•S-4FB (2 x 1mg)
•10X Modification Buffer (1.5 mL)
•10X Conjugation Buffer (1.5 mL)
•10X TurboLINK™
Catalyst Buffer (1.5 mL)
•7kDa MWCO, 0.5 mL Thermo
Scientific™ Zeba™
Column (4)
•Anhydrous DMF (1.5 mL)
•2-Hydrazinopyridine (2-HP) (0.5
mL)
•2-Sulfobenzaldehyde (2-SB) (0.5
mL)
•7kDa MWCO 2 mL Zeba™
Column (2)
•10X PBS (1.5 mL)
•2 mL Collection Tube (12)
•Oligo Resuspension Solution (1.0
mL)
Our Company
Supporting
scientific industries worldwide for over 45 years, Vector Laboratories is
recognized as a pioneer and market leader in immunohistochemistry,
immunofluorescence, glycobiology, and bioconjugation products. As the first
company to commercialize avidin-biotin enzyme complex kits for
immunohistochemistry and antifade mounting media for immunofluorescence, Vector
Laboratories has since introduced over 600 reliable reagents and kits.
Our
product catalog includes VECTASHIELD® Mounting
Media, VECTASTAIN® ABC
kits, ImmPRESS® Polymer
Detection kits, TrueVIEW® Autofluorescence
Quenching Kits, ImmPACT® Enzyme
Substrates, SoluLINK® bioconjugation
chemistry, and a broad range of lectin and glycobiology reagents. These
time-tested tools and methods are used by disease and therapeutics researchers
globally to accelerate their lab processes and improve productivity. Vector
Laboratories’ products have been cited in more than 350,000 scientific
publications and are included in Standard Operating Procedures in labs
worldwide.
