Fab-TACS^® - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag^® technology. CD3 Fab-TACS^® Agarose Column Starter Kit allows label-free isolation of human CD3⁺ T cells from whole blood, buffy coat, PBMCs or other cell suspensions. CD3 is a co-receptor that associates with the T cell receptor and plays a role in T cell activation.

Strep-Tactin^® TACS Agarose columns are filled with Strep-Tactin^® coated agarose macrobeads developed for cell isolation. Low affinity Fab fragments fused to a Twin-Strep-tag^® (Fab-Streps) bind to the matrix. Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to a surface marker. Non-target cells are efficiently washed away. In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps. After elution, the Fab-Streps self-dissociate from the cell surfaces due to their low affinity. The label-free authentic target cells are now ready for further downstream applications.

Advantages:
• Highly pure cells 
• Reversible binding to the target
• Highly viable, non-activated cells
• Label-free cells due to low affinity Fab fragments

The kit contains:
• Strep-Tactin^® TACS Agarose columns , 1 ml (2x)
• CD3 Fab-Strep, human
• 10x Buffer CI
• Biotin stock solution
• TACS Column Adapter

For total cell numbers below 1 x 10⁸, we recommend our Strep-Tactin^® Magnetic Microbeads for free scalability.

Specifications

(주)엘바이오시스템은 Iba Lifesciences의 한국독점대리점입니다.

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