ImmunoStar’s 5-HT (Serotonin) antibodies were engineered by Mark Brownfield, PhD, Associate Professor, Department of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin, Madison. His panel of highly-regarded serotonin antibodies is exclusive to ImmunoStar and have earned a reputation among researchers as being the Serotonin antibodies that work.
Dr. Brownfield’s work on the serotonergic system and its impact on brain function has focused on the molecular anatomy of serotonergic neurons and their target neurons. His antibodies against the serotonergic system includes the antisera to serotonin precursor (5-hydroxytryptophan), primary metabolite (5-hydroxyindole acetic acid), antibodies to serotonin transporter (the target of a class of antidepressant drugs), and five serotonin receptors (5HT1a, 5HT2a, 5HT2c, 5HT5a, and 5HT7).
Dr. Brownfield’s 5HT (Serotonin) studies have shown a correlation between the serotonergic system and circuits important in neuroendocrine, autonomic, emotional, circadian, visual, and motor systems.
Publications – Search our Publications database for 5-HT Serotonin research by antibody name, number, author, species, and other keywords.
We at ImmunoStar are deeply grateful for Dr. Mark Brownfield’s generous services and contribution to the science community over the decades. Our mission is to provide the highest of quality primary antibodies and Mark did just that. It is our great pleasure to carry out Dr. Mark Brownfield’s quality antibodies and continue to share his work with the world.
ImmunoStar’s
5-HT (Serotonin) Antibodies
Description
The antibody produces moderate labeling of raphe neurons in normal rat. In rats whose serotonergic system has been activated, staining intensity is increased to a maximum label. Recommended dilution of the antiserum is 1/4000-1/8000 for biotin-streptavidin/HRP technique.
The specificity of the antiserum was evaluated using a model system of gelatin-indole plugs by a method similar to published procedures (Schipper and Tilders, 1983). Results showed that the 5-HIAA antibody dose dependently stained 5-HIAA but did not stain any concentration of 5-HT or 5-HTP. The antiserum was also tested by pre-adsorption at 25 µg/mL with various BSA conjugates. While pre-adsorption with 5-HIAA conjugate completely eliminates immunolabeling, pre-adsorption with conjugates of 5-HT,5-HTP and dopamine had no effect on staining intensity or distribution of stain.
Photo Description: IHC image of neurons staining for 5-H1AA in the raphe nucleus of the rat brainstem. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Host: Rabbit
Quantity/Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: : Rat, Tritonia Diomedea (Sea Slug)
Alternate Names: anti-5-HIAA
RRID: AB_572208
Immunogen: 5-HIAA
Gene Symbol: SLC6A4
Entrez Gene ID: 6532
NCBI Gene Aliases: 5-HTT,5-HTTLPR,5HTT,HTT,OCD1,SERT,hSER
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