CKI alpha Mastery

Harness the power with our antibody

Casein kinase 1 alpha (CK1α) is a member of a multi-isoform family of kinases that are involved in several processes in the brain, from Circadian rhythms and Wnt signaling to DNA repair and transcription and even fast synaptic transmission mediated by glutamate, the major excitatory neurotransmitter in the brain^(1,2). It is also a major kinase responsible for the pathological hyperphosphorylation of tau protein in the paired helical filaments (PHFs) found in the brain tissue of AD patients⁽³⁾.

Our antibody, derived from the smallest CKIα isotype, effectively interacts with all CKIα isotypes, exhibiting specificity exclusively for CKIα isoforms, including its four variations. It doesn't cross-react with other members of the CKI family, such as CKI beta, gamma, delta or epsilon proteins. This antibody is suitable for various application, including WB, ICC, IHC-Frozen. 

References:

(1) Jiang, S., et al., Cell Commun Signal 16, 23 (2018), PMC5968562 (CK1 alpha review paper).

(2) Chergui, K. et al.,J Neurosci. 2005 Jul 13; 25(28): 6601–6609, PMC6725422 (glutamate fast transmission role ref)

(3) Kuret, J et al.,J Neurochem.1997 Dec;69(6):2506-15, PMID: 9375684 (PHF AD disease ref) 

A:Immunofluorescence: Visualize CKIα expression (red) in HeLa cells using

C-1371-100. Nuclear pore complexes (green) were labeled with M-1397-250 antibody, and cell nuclei were highlighted 

with Hoechst dye (blue). B: Western Blot: C-1371-100 (1:10,000 dilution) detects 38 kDa and 34 kDa bands, 

corresponding to CK1 alpha and related isotypes, in whole rat spinal cord homogenate.

Explore the complexities of CKIα in your research using this versatile antibody

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