PURIFICATION PROCEDURE OF STREP-TACTIN® AND STREP-TACTIN®XT
A comparison of purification via Strep-Tactin® and Strep-Tactin®XT depicts two changes in the procedure. The first and second step
(lysate application & wash) remain the same. But the elution and the regeneration streps are different for both systems. For elution from
Strep-Tactin® desthiobiotin is used whereas Strep-Tactin®XT requires biotin for elution. Also the regeneration step differs. HABA is used
for regeneration from Strep-Tactin® and in case of Strep-Tactin®XT 10 mM NaOH is applied.