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[iba] Strep-Tactin® vs Strep-Tactin®XT - purification procedure 차이

관리자 2019.02.13 17:05 조회 114

PURIFICATION PROCEDURE OF STREP-TACTIN® AND STREP-TACTIN®XT
A comparison of purification via Strep-Tactin® and Strep-Tactin®XT depicts two changes in the procedure. The first and second step
(lysate application & wash) remain the same. But the elution and the regeneration streps are different for both systems. For elution from
Strep-Tactin® desthiobiotin is used whereas Strep-Tactin®XT requires biotin for elution. Also the regeneration step differs. HABA is used
for regeneration from Strep-Tactin® and in case of Strep-Tactin®XT 10 mM NaOH is applied.

strep 1.PNG