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[iba] Launch Protein A Agarose

관리자 2019.02.13 17:22 조회 2116 추천 25

Protein A

As the developer of high quality tools for recombinant protein production, we have extended our product portfolio by Protein A Agarose affinity resins.

Protein A is commonly used for the affinity purification of IgG class antibodies. It binds to immunoglobulins through interaction with the IgG heavy chain within the Fc region and purifies all antibodies of the target class without regard to antigen specificity. Antibodies from serum (polyclonal antibodies), ascites or cell culture supernatant of hybridoma cell lines (monoclonal antibodies) can thus be easily isolated.

IBAs' Protein A provides

High NaOH stability (CIP 0.5 M NaOH 100 Cycles)
≥70 mg/ml binding capacity
Cost effective products

Now 25% off introductory price*!

Order your free sample here!

*Discount is valid until March 31^st 2019. Not valid in combination with other IBA discounts. Distributors and IBA staff excluded.

Product shop

Product	Contents	Cat.no	Price	Order
Protein A Agarose 50% suspension; 1 ml	1 ml	6-2010-001	EUR 39,00	Quantity:
25% off introductory price until March 31st, 2019. Not valid in combination with other IBA discounts. IBA staff excluded. ... Further details, manuals, data sheets, related products and more
Protein A Agarose 50% suspension; 3 ml	3 ml	6-2010-002	EUR 89,00	Quantity:
25% off introductory price until March 31st, 2019. Not valid in combination with other IBA discounts. IBA staff excluded. ... Further details, manuals, data sheets, related products and more
Protein A Agarose 50% suspension; 10 ml	10 ml	6-2010-005	EUR 229,00	Quantity:
25% off introductory price until March 31st, 2019. Not valid in combination with other IBA discounts. IBA staff excluded. ... Further details, manuals, data sheets, related products and more
Protein A Agarose 50% suspension; 50 ml	50 ml	6-2010-025	EUR 909,00	Quantity:
25% off introductory price until March 31st, 2019. Not valid in combination with other IBA discounts. IBA staff excluded. ... Further details, manuals, data sheets, related products and more
Protein A Agarose 50% suspension; 200 ml	200 ml	6-2010-100	EUR 2.909,00	Quantity:
25% off introductory price until March 31st, 2019. Not valid in combination with other IBA discounts. IBA staff excluded. ... Further details, manuals, data sheets, related products and more
Gravity flow Protein A Agarose column; 0,5 ml	1 x 0,5 ml	6-2015-001	EUR 29,00	Quantity:
25% off introductory price until March 31st, 2019. Not valid in combination with other IBA discounts. IBA staff excluded. ... Further details, manuals, data sheets, related products and more
Protein A Agarose cartridge; 1 ml	1 x 1 ml	6-2021-001	EUR 139,00	Quantity:
25% off introductory price until March 31st, 2019. Not valid in combination with other IBA discounts. IBA staff excluded. ... Further details, manuals, data sheets, related products and more
Protein A Agarose cartridge; 5 ml	1 x 5 ml	6-2022-001	EUR 549,00	Quantity:
25% off introductory price until March 31st, 2019. Not valid in combination with other IBA discounts. IBA staff excluded. ... Further details, manuals, data sheets, related products and more

Technical specifications

Dynamic binding capacity (DBC 10%) of IBA Protein A determined with a monoclonal antibody.

DBC of Protein A remains stable after treatment with 0.5 M NaOH over~100 cycles. 1 cycle = 15 min.

Data

Capacity* (DBC 10%)	≥70 mg hu IgG/mL resin at residence time 6 min ≥50 mg hu IgG/mL resin at residence time 2.4 min ≥25 mg hu IgG/mL resin at residence time 1 min
Flow rate	0.16 - 1 mL/min (1 mL cartridge) 0.83 - 5 mL/min (5 mL cartridge) 250 cm/h (50% suspension)
CIP (0.5 M NaOH 100 Cycles)	No significant change in performance after 100 cycles with 0.5 M NaOH (1 cycle = 15 min contact time) use at room temperature
Particle size	45 µm

*Dynamic binding capacity (DBC 10%) was determined with 1 mg/ml monoclonal hu IgG1 using a 1 mL column at 1, 2.4 and 6 minutes residence time.

Protein A does not bind to all IgG's equivalently and binding varies among species. Even within a species, Protein A interacts with some subclasses of IgG and not others.

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