Products for Immuno-Colocalization
Products for Live Cell Flow Cytometry
We put in tremendous effort in designing antibodies that recognize extracellular domains of proteins.
With Alomone Labs extracellular antibodies:
No need for cell permeabilization and fixation Cell surface detection of proteins
Products for Live Cell Imaging (LCI)
Alomone Labs is committed to developing cutting edge reagents for visualizing ion channels in live cell imaging (LCI) experiments. For this purpose we have established a line of antibodies recognizingextracellular epitopes of various ion channels. Below are new antibodies ideal for LCI.
Labeled Toxins as Probes for Detecting Ion Channels
A Bit of Color Goes a Long Way
Alomone Labs MmTx1 Toxin-ATTO-488 labels GABA(A) α1 receptor in rat cerebellum:
Alomone Labs MmTx1 Toxin ATTO-488 binds to rat cerebellum:
We gladly take on collaboration projects. Please contact us.
Ion Channel Products in Featured Papers
A Potential Role for Kir5.1 Channel in pH Sensitivity in 5-HT Neurons
Serotonin (5-HT) neurons in the brain stem play an important role in maintaining/modulating ventilation and pH sensitivity for proper functioning of the neural network.
A search of age-related changes of pH-sensitive molecules in 5-HT neurons gave rise to the identification of Kir5.1 channel as a potential candidate in CO2/pH chemosensitivity in brainstem 5-HT neurons. Immunohistochemical staining of rat brainstem sections using Anti-Kir4.1 (KCNJ10) Antibody (#APC-035) and Anti-Kir5.1 Antibody (#APC-123) showed that both channels are expressed (green). However, Kir4.1 staining is limited to astrocytes, while that of Kir5.1 is detected in 5-HT neurons (Figure 1).
The authors do not rule out the possibility that other proteins, namely pH-sensitive ion channels may also be involved.
Figure 1. Expression of Kir4.1 and Kir5.1 channels in rat brainstem:
Adapted from Puissant, M.M. et al. (2017) Front. Cell. Neurosci. 11, 34. with permission of Frontiers.